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Learn how the Benedict’s test helps detect reducing substances in urine, including glucose, galactose, and fructose. Understand its chemical basis, clinical uses in metabolic disorders, and how to interpret results effectively. Read More
Learn how to design PCR/Sanger sequencing primers using Ensembl and NCBI Primer-BLAST with a real-life example. A complete step-by-step guide for genetics labs and clinicians. Read More
A complete guide to PCR and sequencing primer design: length, Tm, GC content, dimers, specificity, and software tools—made simple and practical. Read More
Agarose gel electrophoresis is a widely used laboratory technique for the separation and analysis of DNA fragments. This method exploits the negative charge of DNA, which allows it to migrate through a gel matrix under the influence of an electric field. The technique not only enables the visualization of DNA but also allows researchers to... Read More
Extracting DNA is one of the most fundamental techniques in molecular biology. Whether you’re studying human genetics, diagnosing inherited diseases, or performing forensic analysis, the first step is always the same: isolating high-quality, intact DNA. One of the most reliable and cost-effective techniques for this is the Salting Out Method. This article walks you through... Read More