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Learn the complete process of Polymerase Chain Reaction (PCR) — from its principle, steps, and components to real-world amplification limits, enzyme fidelity, and PCR enhancers like DMSO, betaine, and glycerol. Read More
Learn how RFLP and STR-based polymorphic markers are used in genetic diagnostics. Explore real-world applications like sickle cell testing and maternal cell contamination detection, and why older techniques like RFLP still hold value today. Read More
Learn how to use SYBR Green qPCR to detect heterozygous gene deletions. Step-by-step guide with data analysis, interpretation, and troubleshooting tips for polymorphic dropout. Read More
Learn how to validate heterozygous gene deletions using SYBR Green-based qPCR. Step-by-step guide with melt curve analysis, and ΔCt/ΔΔCt interpretation. Read More