Posts in Lab Techniques

Polymerase Chain Reaction: Principle, Steps, Components, and Enhancers Explained

Learn the complete process of Polymerase Chain Reaction (PCR) —[…]

Decoding DNA with RFLP and STR Markers

Learn how RFLP and STR-based polymorphic markers are used in[…]

Diagnosis of Spinal Muscular Atrophy (SMA) Using DraI-based PCR-RFLP

Learn how Spinal Muscular Atrophy (SMA) can be diagnosed using[…]

Fast, Accurate, and Fluorescent: Using QF-PCR to Decode Sex Chromosome Disorders

Learn how QF-PCR helps detect sex chromosome aneuploidies like Turner[…]

SYBR Green-Based qPCR Protocol with Detailed Explanation of Melt Curve and Data Analysis

Learn how to validate heterozygous gene deletions using SYBR Green-based[…]

Understanding Spectrophotometry Using NanoDrop 2000: Measuring DNA Concentration and Purity

Learn how to use the NanoDrop 2000 spectrophotometer to accurately[…]

Urine Reducing Substances: A Quick Bedside Clue Using Benedict’s Test

Learn how the Benedict’s test helps detect reducing substances in[…]

Agarose Gel Electrophoresis: Step-by-Step Protocol with Troubleshooting Tips

Agarose gel electrophoresis is a widely used laboratory technique for[…]